[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"$fWUhYx7mN27x3ZwWFhkZ6cM7-ob8uJ9nVb_QBopfycl8":3},{"answer":4,"createTime":5,"id":6,"options":7,"origin":12,"question":19,"related":20,"source":31,"type":81},[],"2024-10-27 23:18:01",163407215,[8,9,10,11],"繁琐的劳动","高昂的费用","同源重组概率低","中靶ES细胞要凑巧发育成生殖细胞且刚好成功受精的机率很低",{"count":13,"courseId":14,"courseImg":15,"courseName":16,"workId":17,"workName":18},14,"b8a88b9fa45f3eacc05d4dc03140e889","https:\u002F\u002Ftihai-oss-cloud.itihey.com\u002Fimg\u002F8f6d1861c2d7225ca42808692d75026e.jpg","基因工程","work_38004443","第九章 \b作业","传统基因敲除的不足有哪些",[21,33,42,51,60,69,78,82,90,95],{"answer":22,"createTime":23,"id":24,"options":25,"question":30,"source":31,"type":32},[],"2024-10-27 23:14:07",163406575,[26,27,28,29],""如来神掌"",""小李飞刀"",""上古神器"",""大力水手"","分子生物学界把CRISPR比喻成什么","v1",0,{"answer":34,"createTime":23,"id":35,"options":36,"question":41,"source":31,"type":32},[],163406576,[37,38,39,40],"Cre-loxp 系统","TALEN打靶","CRISPR CAS9","RNA干扰","分子生物学界\b被喻为"小李飞刀"的是什么",{"answer":43,"createTime":23,"id":44,"options":45,"question":50,"source":31,"type":32},[],163406577,[46,47,48,49],"DNA与DNA相互作用","DNA与RNA相互作用","RNA与RNA相互作用","蛋白与RNA相互作用","RNAi序列识别是通过什么实现的",{"answer":52,"createTime":23,"id":53,"options":54,"question":59,"source":31,"type":32},[],163406578,[55,56,57,58],"是否能够成功多半取决于研究者的技术和水平","传统的基因敲除付出的时间相当漫长,劳动繁琐、费用高昂","传统的基因敲除可以用受精卵细胞和ES细胞,所以效率是很高的","Cre-loxp系统下的条件基因敲除小鼠效率很高,所以它不是传统的基因敲除技术范畴","关于传统基因敲除\b描述\b正确的是哪个",{"answer":61,"createTime":23,"id":62,"options":63,"question":68,"source":31,"type":32},[],163406579,[64,65,66,67],"当两个loxp位点反向位于两条链上时,它所起的作用是让两条链上的序列互换","当两个loxp位点同向位于两条链上时,它所起的作用是让两条链上的序列互换","当两个loxp位点同向位于一链上时,可以切除 两个loxp位点中间的基因序列","当两个loxp位点反向位于一链上时,可以使\b两个loxp位点中间的基因序列倒置","在Cre-loxp 系统的三种模式中,下列哪个是\b错误的",{"answer":70,"createTime":23,"id":71,"options":72,"question":77,"source":31,"type":32},[],163406580,[73,74,75,76],"比方说EcoR1识别GAATTC序列,但是这段序列理论上每隔4的6次方个碱基就会出现一次,即每隔4096个碱基就会有一个位点,它几乎会使每个基因都被切断","限制性内切酶只能切割特定的序列,并不一定能切目的基因","限制性内切酶不能随意进入靶细胞中行使切割功能","限制性内切酶的mRNA不能在靶细胞中正确翻译出有活性的内切酶","在新型打靶技术中,要能特异性识别DNA并切断它,限制性内切酶\b为什么不能用来切断DNA",{"answer":79,"createTime":5,"id":6,"options":80,"question":19,"source":31,"type":81},[],[8,9,10,11],1,{"answer":83,"createTime":5,"id":84,"options":85,"question":88,"source":31,"type":89},[],163407216,[86,87],"正确","错误","条件基因敲出中的"条件"是指特定的时间和特定的细胞",3,{"answer":91,"createTime":5,"id":92,"options":93,"question":94,"source":31,"type":89},[],163407217,[86,87],"Cre-loxp 系统是指Cre重组酶,只要发现在任何一条DNA链上存在两个34个碱基长度反向重复序列(命名为LOXP位)点,不管这两个loxp位点是正向还是反向,都可以切去中间的DNA序列",{"answer":96,"createTime":5,"id":97,"options":98,"question":99,"source":31,"type":89},[],163407218,[86,87],"DNA断点处的同源重组率增高,以及非同源重组修复错误,可以帮助我们改造特定的基因"]