[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"$fy85IdVUTSvS2e62K89nH5yEN6tI9hv0X4LLm4t3ylko":3},{"answer":4,"createTime":5,"id":6,"options":7,"origin":12,"question":19,"related":20,"source":31,"type":104},[],"2025-04-26 19:45:10",184745247,[8,9,10,11],"Proteins are first separated by isoelectric focusing in a thin strip gel","The gel is then laid horizontally on a second, slab-shaped gel, and the proteins are separated by SDS polyacrylamide gel electrophoresis","Horizontal separation reflects differences in pI; vertical separation reflects differences in molecular weight. The original protein complement is thus spread in two dimensions","Thousands of cellular proteins can be resolved using this technique. Individual protein spots can be cut out of the gel and identified by mass spectrometry",{"count":13,"courseId":14,"courseImg":15,"courseName":16,"workId":17,"workName":18},125,"ecb63186fd2348dd972900f765a15722","https:\u002F\u002Ftihai-oss-cloud.itihey.com\u002Fimg\u002Fe91b8b9d3b83a0d6a9d655b826214095.jpg","Biochemistry 《生物化学》一 专业基础课（全英教学）","e9ae3d8bd84d4fb4a587a369705d2aad","Test 3 - Lecture 3","What is the procedure of two-dimensional electrophoresis",[21,33,42,47,56,65,74,83,90,95],{"answer":22,"createTime":23,"id":24,"options":25,"question":30,"source":31,"type":32},[],"2025-04-26 19:45:09",184745186,[26,27,28,29],"mass or 3D structure","mass or charge","3D structure or charge","mass or 2D structure","Electrophoresis separates proteins on the basis of _________________","v1",0,{"answer":34,"createTime":23,"id":35,"options":36,"question":41,"source":31,"type":32},[],184745187,[37,38,39,40],"2","3","4","5","____ cysteines form a cystine by reversible formation of a disulfide bond via oxidation",{"answer":43,"createTime":23,"id":44,"options":45,"question":46,"source":31,"type":32},[],184745188,[37,38,39,40],"Desmosine is formed from ___ lysine residues, which is found in the fibrous protein elastin",{"answer":48,"createTime":23,"id":49,"options":50,"question":55,"source":31,"type":32},[],184745189,[51,52,53,54],"consensus","signature","repeat","nonconsensus","When a series of related nucleic acid or protein sequences are compared, a ____________ sequence is the one that reflects the most common base or amino acid at each position",{"answer":57,"createTime":23,"id":58,"options":59,"question":64,"source":31,"type":32},[],184745190,[60,61,62,63],"Acetylation","Phosphorylation","Glycosylation","Prenylation","_______________ is the most common type of regulatory modification of proteins",{"answer":66,"createTime":23,"id":67,"options":68,"question":73,"source":31,"type":32},[],184745191,[69,70,71,72],"Gly","Gln","Glu","Pro","The one-letter code for 20 amino acids was devised by the American biochemist Margaret Oakley Dayhoff, considered by many to be the founder of the field of bioinformatics. Q is ____",{"answer":75,"createTime":23,"id":76,"options":77,"question":82,"source":31,"type":32},[],184745192,[78,79,80,81],"net electric charge","size","binding affinity","number of amino acid residues","Ion-exchange chromatography separates proteins based on their differences in _____________________",{"answer":84,"createTime":23,"id":85,"options":86,"question":89,"source":31,"type":32},[],184745193,[87,88,37,38],"0","1","When pH = pI, the net charge of a protein is _____",{"answer":91,"createTime":23,"id":92,"options":93,"question":94,"source":31,"type":32},[],184745194,[88,37,38,39],"After removal of the precursor signal peptide, human proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. So human insulin contains ____ chains",{"answer":96,"createTime":23,"id":97,"options":98,"question":103,"source":31,"type":32},[],184745195,[99,100,101,102],"Leu","Lys","Arg","His","The one-letter code for 20 amino acids was devised by Margaret Oakley Dayhoff, considered by many to be the founder of the field of bioinformatics. K is ________",1]