[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"$fn5JG_YVOinuS0THGSxSeJMbiot5k4KlJdgZ0DrLZSH0":3},{"answer":4,"createTime":5,"id":6,"options":7,"origin":13,"question":20,"related":21,"source":32,"type":33},[],"2025-05-25 18:04:11",190728360,[8,9,10,11,12],"They decrease the electrical charge of the molecules","They work like a sieve, allowing smaller molecules to move faster than larger molecules","They increase the electrical charge of the molecules","They allow all molecules to move at the same speed","They prevent molecules from moving",{"count":14,"courseId":15,"courseImg":16,"courseName":17,"workId":18,"workName":19},107,"e0d5dfbf0a1edbb3197aba4c82a0e577","https:\u002F\u002Ftihai-oss-cloud.itihey.com\u002Fimg\u002F48eba3395437dd881b1b9275a0ad1028.png","Applied Electrophoresis","work_43577260","Electrophoresis Review 2025","What is the effect produced by the pores in the gel during electrophoresis",[22,34,43,52,61,64,74,83,92,102],{"answer":23,"createTime":5,"id":24,"options":25,"question":31,"source":32,"type":33},[],190728356,[26,27,28,29,30],"It indicates the protein is degraded","It indicates the presence of multiple subunits","It indicates the protein is not denatured","It indicates the protein is a complex","It indicates the protein is pure","What is the significance of the single band observed for each protein in SDS-PAGE","v1",0,{"answer":35,"createTime":5,"id":36,"options":37,"question":42,"source":32,"type":33},[],190728357,[38,39,40,41],"A room at 30°C","A 4°C refrigerator","A cool and dark place at room temperature","A well-ventilated place at 25°C","When storing the Tetramethylethylenediamine (TEMED) stock solution, which of the following environments is the least conducive to its storage",{"answer":44,"createTime":5,"id":45,"options":46,"question":51,"source":32,"type":33},[],190728358,[47,48,49,50],"Make the staining more uniform","Accelerate the staining speed","Have no impact on the staining effect","Cause the gel to break","When staining the gel with Coomassie Brilliant Blue R-250, if it is shaken vigorously, it may",{"answer":53,"createTime":5,"id":54,"options":55,"question":60,"source":32,"type":33},[],190728359,[56,57,58,59],"The solution has a slight turbid and is stored at room temperature","The color of the solution does not change, stored at 4°C.","The solution has no odor, stored at room temperature","The solution is clear and transparent, stored at room temperature","During the storage of 1.5 mol\u002FL Tris-HCl (pH 8.8) solution, if the following situation occurs, it indicates that the operation or storage may be incorrect",{"answer":62,"createTime":5,"id":6,"options":63,"question":20,"source":32,"type":33},[],[8,9,10,11,12],{"answer":65,"createTime":5,"id":66,"options":67,"question":73,"source":32,"type":33},[],190728361,[68,69,70,71,72],"It stains the molecules","It denatures the proteins","It provides a source of ions to carry the current","It stabilizes the gel","It changes the pH of the gel","What is the role of the buffer in electrophoresis",{"answer":75,"createTime":5,"id":76,"options":77,"question":82,"source":32,"type":33},[],190728362,[78,79,80,81],"Preparing it fresh before use is to ensure its activity","Just add distilled water when using it","After dissolution, it can be stored at 4°C for 1 month","The dry powder can be weighed in appropriate amounts and placed in 1.5 ml EP tubes for later use","Regarding the 10% Ammonium Persulfate (APS) solution, which of the following statements is wrong",{"answer":84,"createTime":5,"id":85,"options":86,"question":91,"source":32,"type":33},[],190728363,[87,88,89,90],"Add a calculated amount of concentrated HCl to adjust the pH without considering the concentration change","Discard the solution and start over from scratch","Evaporate 100 ml of water and then adjust the pH with concentrated HCl","Add an appropriate amount of Tris to double the concentration and then adjust the pH","Suppose during the preparation of a large-scale experiment using 1.0 mol\u002FL Tris-HCl (pH 6.8), you miscalculated the volume of distilled water to be 200 ml instead of 100 ml while keeping the mass of Tris correct. To adjust the solution to the correct concentration and pH, which of the following steps is the most appropriate",{"answer":93,"createTime":5,"id":94,"options":95,"question":101,"source":32,"type":33},[],190728364,[96,97,98,99,100],"8.8 kDa","80000 dalton","8800","10 kDa","not available","The molecular weight of a protein contains 80 amino acids is approximately",{"answer":103,"createTime":5,"id":104,"options":105,"question":111,"source":32,"type":33},[],190728365,[106,107,108,109,110],"Low salt concentration","High salt concentration","No salt required","High temperature","Low temperature","Which factor is essential for the precipitation of nucleic acids in alcohol precipitation"]