[{"data":1,"prerenderedAt":-1},["ShallowReactive",2],{"$fqppV_2G-DA5ggoIHWb2wx-FRmEmoS8xSRk14a7ZgSVE":3},{"answer":4,"createTime":5,"id":6,"options":7,"origin":12,"question":19,"related":20,"source":24,"type":25},[],"2025-11-23 16:52:11",239990341,[8,9,10,11],"依赖限制性内切酶和连接酶","引入酶切位点作为"疤痕"序列","通过同源重组实现DNA片段插入","只能用于小片段克隆",{"count":13,"courseId":14,"courseImg":15,"courseName":16,"workId":17,"workName":18},20,"53e1d2ef4961cca8eea3e23969ad2cb9","https:\u002F\u002Ftihai-oss-cloud.itihey.com\u002Fimg\u002F03a579384a6dc297c89809b582fcc767.png","默认课程","work_46510148","第5次作业",""无缝克隆"技术的主要特点是",[21,26,35,44,53,62,71,80,89,98],{"answer":22,"createTime":5,"id":6,"options":23,"question":19,"source":24,"type":25},[],[8,9,10,11],"v1",0,{"answer":27,"createTime":5,"id":28,"options":29,"question":34,"source":24,"type":25},[],239990342,[30,31,32,33],"Krysan","Alan Coulson","Liu et al","Du et al","图位克隆法最早由谁提出",{"answer":36,"createTime":5,"id":37,"options":38,"question":43,"source":24,"type":25},[],239990343,[39,40,41,42],"需要知道基因的DNA序列","需要知道基因的表达产物","无需知道基因序列和表达产物信息","只能用于模式植物","图位克隆法最大的优点是",{"answer":45,"createTime":5,"id":46,"options":47,"question":52,"source":24,"type":25},[],239990344,[48,49,50,51],"构建遗传图谱","筛选与目标性状连锁的分子标记","直接克隆基因","进行转基因验证","BSA(Bulked Segregant Analysis)常用于",{"answer":54,"createTime":5,"id":55,"options":56,"question":61,"source":24,"type":25},[],239990345,[57,58,59,60],"直接获得目标基因","通过连续筛选克隆逐步接近目标基因","用于构建遗传图谱","用于差异表达分析","染色体步移(Chromosome walking)的目的是",{"answer":63,"createTime":5,"id":64,"options":65,"question":70,"source":24,"type":25},[],239990346,[66,67,68,69],"mRNA差异显示","T-DNA插入突变","同源序列法","EST数据库筛选","以下哪种方法属于"标签法"",{"answer":72,"createTime":5,"id":73,"options":74,"question":79,"source":24,"type":25},[],239990347,[75,76,77,78],"扩增已知全长基因","克隆T-DNA插入侧翼序列","构建物理图谱","进行基因表达分析","TAIL-PCR常用于",{"answer":81,"createTime":5,"id":82,"options":83,"question":88,"source":24,"type":25},[],239990348,[84,85,86,87],"目标基因在染色体上的位置已知","目标基因在其他物种中已有同源序列信息","必须有突变体材料","必须构建cDNA文库","同源序列法克隆基因的前提是",{"answer":90,"createTime":5,"id":91,"options":92,"question":97,"source":24,"type":25},[],239990349,[93,94,95,96],"操作简单,成本低","可检测低丰度mRNA,假阳性率低","不需要mRNA","适用于差异极大的材料","SSH(抑制消减杂交)的主要优点是",{"answer":99,"createTime":5,"id":100,"options":101,"question":106,"source":24,"type":25},[],239990350,[102,103,104,105],"表达序列标签","电子序列标签","酶切序列标签","进化序列标签","EST是指"]