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单选题 The binding energy ______ is a major source of free energy used by enzymes to lower the activation energies of reactions
学科：Biochemistry 《生物化学》一 专业基础课（全英教学）
时间：2025-04-19 09:47:48
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题目1单选题
Chymotrypsin is a ________ proteaseA. cysteineB. serineC. lysineD. threonine
题目2单选题
The metal cofactor for urease is _______A. Cu2+B. Ni2+C. Mn2+D. Fe2+
题目3单选题
An enzyme catalyzes the reaction A ⇌ B. The enzyme is present at a concentration of 1 nM, and the Vmax is 2 μM s-1. The Km for substrate M is 4 μM. What values of Vmax and Km would be observed in the presence of sufficient amounts of an uncompetitive inhibitor to generate an α' of 2.0A. apparent Vmax= 2 μM s-1; apparent Km = 2 μMB. apparent Vmax = 1 μM s-1; apparent Km = 1 μMC. apparent Vmax = 1 μM s-1; apparent Km = 2 μMD. apparent Vmax = 2 μM s-1; apparent Km = 4 μM
题目4单选题
The functional groups transferred by tetrahydrofolate are ___________________A. acyl groupsB. one-carbon groupsC. amino groupsD. electrons
题目5单选题
The protein part of a holoenzyme is called the ______________A. apoenzymeB. semienzymeC. dienzymeD. monoenzyme
题目6单选题
To approximate the concentration of enzymes in a liver cell, assume that the cell contains equal concentrations of 1,000 different enzymes in solution in the cytosol and that each protein has a molecular weight of 100,000. Assume also that the cell is a cylinder (diameter = 1.0 μm, height = 2.0 μm), that the cytosol (specific gravity = 1.20) is 20% soluble protein by weight, and that the soluble protein consists entirely of enzymes. Calculate the average molar concentration of each enzyme in this cellA. 2.4 × 10-4 MB. 2.4 × 10-5 MC. 2.4 × 10-6 MD. 2.4 × 10-7 M
题目7单选题
Assume a reaction follows Michaelis-Menten kinetics. Determine the fraction of Vmax that would be obtained at a substrate concentration that is 10KmA. 0.61B. 0.71C. 0.81D. 0.91
题目8单选题
To approximate the concentration of enzymes in a human cell, assume that the cell contains equal concentrations of 1,000 different enzymes in the cytosol and that each enzyme has a molecular weight of 100,000. Assume also that the cell is a ball (diameter 30 μm), that the cytosol (specific gravity 1.20) is 20% soluble protein by weight, and that the soluble protein consists entirely of enzymes. Calculate the average molar concentration of each enzyme in this hypothetical cellA. 2.4 × 10-4 MB. 2.4 × 10-5 MC. 2.4 × 10-6 MD. 2.4 × 10-7 M
题目9单选题
The binding energy ______ is a major source of free energy used by enzymes to lower the activation energies of reactionsA. △GAB. △GBC. △GCD. △GD
题目10单选题
An enzyme catalyzes the reaction A ⇌ B. The enzyme is present at a concentration of 2 nM, and the Vmax is 1.2 μM s-1. The Km for substrate A is 10 μM. Calculate the initial velocity of the reaction, V0, when the substrate concentration is 30 μMA. 0.7 μM s-1B. 0.8 μM s-1C. 0.9 μM s-1D. 0.95 μM s-1
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