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单选题 Suppose during the preparation of a large-scale experiment using 1.0 mol/L Tris-HCl (pH 6.8), you miscalculated the volume of distilled water to be 200 ml instead of 100 ml while keeping the mass of Tris correct. To adjust the solution to the correct concentration and pH, which of the following steps is the most appropriate
学科：Applied Electrophoresis
时间：2025-05-25 10:04:11
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题目1单选题
What is the significance of the single band observed for each protein in SDS-PAGEA. It indicates the protein is degradedB. It indicates the presence of multiple subunitsC. It indicates the protein is not denaturedD. It indicates the protein is a complexE. It indicates the protein is pure
题目2单选题
When storing the Tetramethylethylenediamine (TEMED) stock solution, which of the following environments is the least conducive to its storageA. A room at 30°CB. A 4°C refrigeratorC. A cool and dark place at room temperatureD. A well-ventilated place at 25°C
题目3单选题
When staining the gel with Coomassie Brilliant Blue R-250, if it is shaken vigorously, it mayA. Make the staining more uniformB. Accelerate the staining speedC. Have no impact on the staining effectD. Cause the gel to break
题目4单选题
During the storage of 1.5 mol/L Tris-HCl (pH 8.8) solution, if the following situation occurs, it indicates that the operation or storage may be incorrectA. The solution has a slight turbid and is stored at room temperatureB. The color of the solution does not change, stored at 4°C.C. The solution has no odor, stored at room temperatureD. The solution is clear and transparent, stored at room temperature
题目5单选题
What is the effect produced by the pores in the gel during electrophoresisA. They decrease the electrical charge of the moleculesB. They work like a sieve, allowing smaller molecules to move faster than larger moleculesC. They increase the electrical charge of the moleculesD. They allow all molecules to move at the same speedE. They prevent molecules from moving
题目6单选题
What is the role of the buffer in electrophoresisA. It stains the moleculesB. It denatures the proteinsC. It provides a source of ions to carry the currentD. It stabilizes the gelE. It changes the pH of the gel
题目7单选题
Regarding the 10% Ammonium Persulfate (APS) solution, which of the following statements is wrongA. Preparing it fresh before use is to ensure its activityB. Just add distilled water when using itC. After dissolution, it can be stored at 4°C for 1 monthD. The dry powder can be weighed in appropriate amounts and placed in 1.5 ml EP tubes for later use
题目8单选题
Suppose during the preparation of a large-scale experiment using 1.0 mol/L Tris-HCl (pH 6.8), you miscalculated the volume of distilled water to be 200 ml instead of 100 ml while keeping the mass of Tris correct. To adjust the solution to the correct concentration and pH, which of the following steps is the most appropriateA. Add a calculated amount of concentrated HCl to adjust the pH without considering the concentration changeB. Discard the solution and start over from scratchC. Evaporate 100 ml of water and then adjust the pH with concentrated HClD. Add an appropriate amount of Tris to double the concentration and then adjust the pH
题目9单选题
The molecular weight of a protein contains 80 amino acids is approximatelyA. 8.8 kDaB. 80000 daltonC. 8800D. 10 kDaE. not available
题目10单选题
Which factor is essential for the precipitation of nucleic acids in alcohol precipitationA. Low salt concentrationB. High salt concentrationC. No salt requiredD. High temperatureE. Low temperature
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